|
KMID : 0545120170270050983
|
|
Journal of Microbiology and Biotechnology
2017 Volume.27 No. 5 p.983 ~ p.989
|
|
|
Enhanced Purification of Recombinant Rat NADPH-P450 Reductase by Using a Hexahistidine-Tag
|
|
Park Hyoung-Goo
Lim Young-Ran
Han Song-Hee
Jeong Da-Bin
Kim Dong-Hak
|
|
|
Abstract
|
|
|
|
NADPH-P450 reductase (NPR) transfers electrons from NADPH to cytochrome P450 and heme oxygenase enzymes to support their catalytic activities. This protein is localized within the endoplasmic reticulum membrane and utilizes FMN, FAD, and NADPH as cofactors. Although NPR is essential toward enabling the biochemical and pharmacological analyses of P450 enzymes, its production as a recombinant purified protein requires a series of tedious efforts and a high cost due to the use of NADP+ in the affinity chromatography process. In the present study, the rat NPR clone containing a 6× Histidine-tag (NPR-His) was constructed and heterologously expressed. The NPR-His protein was purified using Ni2+-affinity chromatography, and its functional features were characterized. A single band at 78 kDa was observed from SDS-PAGE and the purified protein displayed a maximum absorbance at 455 nm, indicating the presence of an oxidized flavin cofactor. Cytochrome c and nitroblue tetrazolium were reduced by purified NPR-His in an NADPH-dependent manner. The purified NPR-His successfully supported the catalytic activities of human P450 1A2 and 2A6 and fungal CYP52A21, yielding results similar to those obtained using conventional purified rat reductase. This study will facilitate the use of recombinant NPR-His protein in the various fields of P450 research.
|
|
|
KEYWORD
|
|
|
NADPH-P450 reductase, cytochrome P450, NADPH, Ni2+-affinity chromatography
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|